Human CD3/CD28 T cell Activation Beads Kit

Reactivity

Human

Application

Cell Activation

Conjugate

Magnetic Beads

Publications

1

Cat no : KMS310

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Validation Data Gallery

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  2.5x10^6 human PBMCs in 0.5mL cell culture media are activated by Human CD3/CD28 T Cell Activation Beads Kit for 3 days at a 1:1 bead to cell ratio. Unstimulated cells (left panel) and stimulated cells (right panel) are stained with fluorescence flow antibodies APC-CD69 and FITC-CD25. Total viable lymphocytes are gated. The activation effect of the Human CD3/CD28 T Cell Activation Beads Kit was tested on human PBMCs from three different donors.

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  2.5x10^6 human CD3+ lymphocytes purified by Human CD3 T Cell Isolation Kit (KMS309) in 0.5mL cell culture media are activated by Human CD3/CD28 T Cell Activation Beads Kit for 3 days at a 1:1 bead to cell ratio. Unstimulated cells (left panel) and stimulated cells (right panel) are stained with fluorescence flow antibodies APC-CD69, PE-CD25 and PB-CD4. Total viable lymphocytes are gated. The activation effect of the Human CD3/CD28 T Cell Activation Beads Kit was tested on human PBMCs from three different donors.

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Product Information

T lymphocytes are activated through MHC class II molecules on antigen-presenting cells (APC). After activation, T cells expand rapidly and secrete various cytokines. The Human CD3/CD28 T cell Activation Beads Kit is designed to activate and expand human T cells. This Kit contains Biotinylated anti-human CD3 and CD28 antibodies, as well as cell culture grade Streptavidin magnetic beads. After Streptavidin magnetic beads are loaded with biotinylated CD3/CD28, they mimic antigen presenting cells and can activate resting T lymphocytes from human PBMCs or purified T lymphocytes. After 2-3 days of activation, magnetic beads can easily be removed by magnet. Further T lymphocytes expansion will require human cytokines for in vitro culture.

Components

KMS310-10:  

  · MS310A-10: 100µL 0.1mg/mL functional grade Biotin-CD3 (clone: OKT3)

  · MS310B-10: 100µL 0.2mg/mL functional grade Biotin-CD28 (clone: 28.2)

  · MS310C-10: 500µL cell culture grade Streptavidin magnetic beads (2x10⁸ beads/mL)

KMS310-100:

  · MS310A-100: 1mL 0.1mg/mL functional grade Biotin-CD3 (clone: OKT3)

  · MS310B-100: 1mL 0.2mg/mL functional grade Biotin-CD28 (clone: 28.2)

  · MS310C-100: 5mL cell culture grade Streptavidin magnetic beads (2x10⁸ beads/mL)

Storage

2-8°C

Storage buffer

Biotin antibody buffer: PBS and 0.5% HSA, pH7.4

Streptavidin bead buffer: PBS and 0.5% HSA, 2mM EDTA, pH7.4

Reactivity

Human 

Beads diameter

2.7 µm

Recommend usage

10µL Biotin-CD3, 10µL Biotin-CD28 and 50µL Streptavidin magnetic beads; mix well and add 30µL buffer for incubation. 100µL antibody-loaded magnetic beads contains 1x10⁷ magnetic particles and is used to activate 1x10⁷ purified human T lymphocytes or human PBMCs in 2mL cell culture media (1:1 bead to cell ratio).

Cited in Article as

KMS310, Human CD3/CD28 T cell Activation Beads Kit, Proteintech, IL, USA

Publications

Application	Title
	Cell Mol Life Sci; BCLAF1 binds SPOP to stabilize PD-L1 and promotes the development and immune escape of hepatocellular carcinoma; Authors - Zongdong Yu