Human CD19 Magnetic Beads

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

Cat no : MS005

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Validation Data Gallery

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  Following depletion of CD19+ cells, supernatant cell suspension was stained with PB450-CD3(clone: HIT3a) and CL647-CD19(clone: SJ25C1) antibodies. CD45 positive cells are gated in the analysis. Left panel: CD3-CD19+ cells before selection. Right panel: CD3-CD19+ cells after depletion. Human CD19 Magnetic Beads are tested using PBMC from three different donors.

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Product Information

CD19 is a 95 kDa, type I transmembrane glycoprotein that belongs to the immunoglobin superfamily. It is expressed by B cells and follicular dendritic cells and regulates B cell lineage commitment during hematopoietic stem cell differentiation. It is observed that 6%-23% human PBMC are CD19 positive. Human CD19 Magnetic Beads can be used for isolation or depletion of human CD19 B lymphocytes from PBMC, whole blood, or other sample types. Following incubation with human CD19 antibody conjugated magnetic beads, the cell sample is placed on a magnet. CD19+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in expansion of cells, but are not suitable for flow cytometry analysis. CD19- cells remain in supernatant and could be used forfurther applications.

Components

MS005-10: 100 µL 10 mg/mL Human CD19 Magnetic Beads

MS005-100: 1 mL 10 mg/mL Human CD19 Magnetic Beads

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Reactivity

Human

Recommend usage

10 µL Human CD19 Magnetic Beads for 1*10⁷ cells

Beads Diameter

2.7 μm

Cited in Article as

MS005, Human CD19 Magnetic Beads, Proteintech, IL, USA