Human CD16 Magnetic Beads

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

Cat no : MS007

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Validation Data Gallery

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  Following cell separation, cell suspension was stained with FITC-CD45(F10-89-4) and CL650-CD16(3G8) antibodies. All viable cells are gated in the analysis. Left panel: CD16+CD45+ cells before selection. Right panel: CD16+CD45+ cells after depletion. Human CD16 magnetic beads are tested using PBMC from three donors.

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Product Information

CD16, which is also known as FcγRIII, is a receptor for the Fcγ portion of IgG and is involved in antibody dependent cellular cytotoxicity. 5%-10% human peripheral blood mononuclear cells (PBMC) express CD16. Human CD16 Magnetic Beads Kit is used for isolation or depletion of human CD16 NK cells/monocytes from PBMC, whole blood, or other sample types. CD16 positive cells and negative cells can be separated by a magnet after short incubation with Human CD16 Magnetic Beads. CD16 positive cells remain attached to magnetic beads after separation and can be used for further molecular analysis or in vitro culture, but not suitable for flow cytometry analysis. CD16 negative cells remain in supernatant and can also be used for any further application.

Components

MS007-10:  100 µL 10 mg/mL human CD16 magnetic beads  

MS007-100: 1 mL 10 mg/mL human CD16 magnetic beads

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Reactivity

Human

Recommend usage

10 µL Human CD16 Magnetic Beads for 1*10⁷ cells

Beads Diameter

2.7 μm

Cited in Article as

MS007, Human CD16 Magnetic Beads, Proteintech, IL, USA