Human CD16 Magnetic Beads Kit

Host / Isotype

Mouse / Mouse IgG1, kappa

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

CloneNo.

3G8

Cat no : KMS006

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Validation Data Gallery

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  Following cell separation, cell suspension was stained with FITC-CD45(F10-89-4) and CL650-CD16(3G8) antibodies. All viable cells are gated in the analysis. Left panel: CD16+CD45+ cells before selection. Right panel: CD16+CD45+ cells after depletion. Human CD16 magnetic beads kit is tested using PBMC from three donors.

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Product Information

CD16, which is also known as FcγRIII, is a receptor for the Fcγ portion of IgG and is involved in antibody dependent cellular cytotoxicity. 5%-10% human peripheral blood mononuclear cells (PBMC) express CD16. Human CD16 Magnetic Beads Kit is used for isolation or depletion of human CD16 NK cells/monocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD16 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD16+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD16- cells remain in supernatant and can also be used for further applications.

Components

KMS006-10:  

  · MS001-10: 100µL 10mg/mL streptavidin magnetic beads  

  · MS65090-10: 100µL 0.1mg/mL Biotin-CD16 (clone: 3G8)  

KMS006-100:  

  · MS001-100: 1mL 10mg/mL streptavidin magnetic beads  

  · MS65090-100: 1mL 0.1mg/mL Biotin-CD16 (clone: 3G8) 

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Reactivity

Human

Recommend usage

10µL Biotin-CD16 antibody and 10µL streptavidin beads for 1*10⁷ cells.

Cited in Article as

KMS006, Human CD16 Magnetic Beads Kit, Proteintech, IL, USA