Human CD16 Magnetic Beads Kit
Host / Isotype
Mouse / Mouse IgG1, kappa
Cat no : KMS006
CD16, which is also known as FcγRIII, is a receptor for the Fcγ portion of IgG and is involved in antibody dependent cellular cytotoxicity. 5%-10% human peripheral blood mononuclear cells (PBMC) express CD16. Human CD16 Magnetic Beads Kit is used for isolation or depletion of human CD16 NK cells/monocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD16 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD16+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD16- cells remain in supernatant and can also be used for further applications.
· MS001-10: 100µL 10mg/mL streptavidin magnetic beads
· MS65090-10: 100µL 0.1mg/mL Biotin-CD16 (clone: 3G8)
· MS001-100: 1mL 10mg/mL streptavidin magnetic beads
· MS65090-100: 1mL 0.1mg/mL Biotin-CD16 (clone: 3G8)
PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide
10µL Biotin-CD16 antibody and 10µL streptavidin beads for 1*107 cells.
Cited in Article as
KMS006, Human CD16 Magnetic Beads Kit, Proteintech, IL, USA