Human CD14 Magnetic Beads

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

Cat no : MS006

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Validation Data Gallery

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  Following cell separation, cell suspension was stained with FITC-CD45(F10-89-4) and CL650-CD14(UCHM1) antibodies. All viable cells are gated in the analysis. Left panel: CD14+CD45+ cells before selection. Right panel: CD14+CD45+ cells after depletion. Human CD14 magnetic beads are tested using PBMC from three donors.

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Product Information

CD14 is a surface antigen expressed on monocyte/macrophage. It cooperates with other proteins to mediate innate immune response to bacterial LPS. 5%-10% human peripheral blood mononuclear cells (PBMC) express CD14. Human CD14 Magnetic Beads Kit is used for isolation or depletion of human CD14 monocytes/macrophages from PBMC, whole blood, or other sample types. CD14 positive cells and negative cells could be separated after short incubation with Human CD14 Magnetic Beads on a magnet. CD14 positive cells remain attached to magnetic beads after separation and can be used for further molecular analysis or in vitro culture, but not suitable for flow cytometry analysis. CD14 negative cells remain in the supernatant and can be used also.

Components

MS006-10:  100 µL 10 mg/mL human CD14 magnetic beads  

MS006-100: 1 mL 10 mg/mL human CD14 magnetic beads  

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Reactivity

Human

Recommend usage

10 µL Human CD14 Magnetic Beads for 1*10⁷ cells

Beads Diameter

2.7 μm

Cited in Article as

MS006, Human CD14 Magnetic Beads, Proteintech, IL, USA