Human OX40/CD134 ELISA Kit
Cat no : KE00209
Synonyms
ACT35, ACT35 antigen, CD134, OX40, OX40L receptor, TNFRSF4, TXGP1L
Validation Data Gallery
Product Information
KE00209 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The OX40/CD134 ELISA kit is to be used to detect and quantify protein levels of endogenous OX40/CD134. The assay recognizes human OX40/CD134. An antibody specific for OX40/CD134 has been pre-coated onto the microwells. The OX40/CD134 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for OX40/CD134 is added to detect the captured OX40/CD134 protein.For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human OX40/CD134 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 5.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7293 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 84%-110% |
| Human plasma | 107% | 98%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 79.1 | 2.8 | 3.6 |
| 2 | 20 | 290.5 | 9.1 | 3.1 |
| 3 | 20 | 1,270.6 | 67.0 | 5.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 68.9 | 5.8 | 8.4 |
| 2 | 24 | 278.3 | 25.4 | 9.1 |
| 3 | 24 | 1,144.6 | 59.9 | 5.2 |
Background Information
OX40 (CD134) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, OX40 is activated by its cognate ligand OX40L (CD134L) and functions as a T cell co-stimulatory molecule. OX40-OX40L interactions have been proposed as a potential therapeutic target for treating autoimmunity. OX40 can interact with TRAF2, TRAF3, and TRAF5. In addition to a membrane bound form, OX40 also exists as a soluble form, released by activated lymphocytes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ACT35, ACT35 antigen, CD134, OX40, OX40L receptor, TNFRSF4, TXGP1L |