Human CD11b Magnetic Beads

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

Cat no : MS008

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Validation Data Gallery

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  Following cell separation, cell suspension was stained with FITC-CD45(F10-89-4) and PE-CD11b(ICRF44) antibodies. All viable cells are gated in the analysis. Left panel: CD11b+CD45+ cells before selection. Right panel: CD11b+CD45+ cells after depletion. Human CD11b magnetic beads are tested using PBMC from three donors.

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Product Information

CD11b is the α-chain of integrin receptor CD11b/CD18 (also known as αMβ2, Mac-1, and CR3), is highly expressed on the surface of innate immune cells, including macrophages, neutrophils, eosinophils and basophils. Human CD11b Magnetic Beads Kit is used for isolation or depletion of human CD11b myeloid lineage cells from PBMC, whole blood, or other sample types. CD11b positive cells and negative cells could be separated after short incubation with Human CD11b Magnetic Beads on a magnet. CD11b positive cells remain attached to magnetic beads after separation and can be used for further molecular analysis or in vitro culture, but not suitable for flow cytometry analysis. CD11b negative cells remain in the supernatant and can be used also.

Components

MS008-10:  100 µL 10 mg/mL human CD11b magnetic beads  

MS008-100: 1 mL 10 mg/mL human CD11b magnetic beads 

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide 

Reactivity

Human

Recommend usage

10 µL Human CD11b Magnetic Beads for 1*10⁷ cells

Beads Diameter

2.7 μm

Cited in Article as

MS008, Human CD11b Magnetic Beads, Proteintech, IL, USA