Human ACOX1 ELISA Kit
Cat no : KE00422
Synonyms
ACOX1,ACOX,ACOX 1,AOX,EC:1.3.3.6
Validation Data Gallery
Product Information
KE00422 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ACOX1 ELISA kit is to be used to detect and quantify protein levels of endogenous human ACOX1. The assay recognizes human ACOX1. An antibody specific for human ACOX1 has been pre-coated onto the microwells. The human ACOX1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ACOX1 is added to detect the captured human ACOX1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ACOX1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 51 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 111% | 104%-117% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.91 | 0.17 | 3.51 |
| 2 | 20 | 2.35 | 0.18 | 7.66 |
| 3 | 20 | 0.57 | 0.04 | 0.04 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.89 | 0.21 | 4.31 |
| 2 | 24 | 2.25 | 0.23 | 10.02 |
| 3 | 24 | 0.57 | 0.04 | 6.93 |
Background Information
Acyl-coenzyme A oxidase 1 (ACOX1), the first rate-limiting enzyme for fatty acid β-oxidation (FAO), is a highly conserved enzyme and is mainly expressed in the liver, followed by the kidney, brain, and adipose tissue. FAO takes place in mitochondria and peroxisomes , and its abnormalities are associated with tumorigenesis. Peroxisomes are ubiquitous organelles in eukaryotic cells involved in several metabolic pathways, such as FAO, bile acid synthesis, and purine degradation, etc. As performing the first step of peroxisomal β-oxidation, which is the major enzymatic step, an aberrant expression of ACOX1 has been implicated in a variety of cancers. The upregulation of ACOX1 in lymphoma promotes cancer cell proliferation, while its downregulation inhibits proliferation and induces apoptosis. Knocking down ACOX1 promotes lipid hydrolysis by inducing autophagy in the liver, thereby preventing hepatic steatosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ACOX1,ACOX,ACOX 1,AOX,EC:1.3.3.6 |