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Recombinant Human ACE2 protein (His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 44-176 ng/mL

Cat no : Eg0074

Validation Data Gallery

  • Purity of Recombinant Human ACE2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human ACE2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human ACE2 (His tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (hFc tag, Myc tag, His tag) with a linear range of 44-176 ng/mL.

    Immobilized Human ACE2 (His tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (hFc tag, Myc tag, His tag) with a linear range of 44-176 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human ACE2 (His tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (hFc tag, Myc tag, His tag) with a linear range of 44-176 ng/mL.
Expression HEK293-derived Human ACE2 protein Gln18-Ser740 (Accession# Q9BYF1) with a His tag at the N-terminus.
GeneID 59272
Accession Q9BYF1
PredictedSize 84.4 kDa
SDS-PAGE 85-105 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Angiotensin-converting enzyme 2 (ACE2), a critical regulator of the renin-angiotensin system (RAS), belongs to the peptidase M2 family. It plays an important role in cardiovascular homeostasis by regulating vascular tone, fluid, and electrolyte balance. ACE2 functions as a carboxymonopeptidase hydrolyzing the cleavage of a single C-terminal residue from Angiotensin-II (Ang-II), the key peptide hormone of RAS, to form Angiotensin-(1-7) (Ang-(1-7)), which binds to the G-protein-coupled Mas receptor and activates signaling pathways that counteract the pathways activated by Ang-II. ACE2 is expressed in a variety of tissues, including the kidneys, testes, heart, and intestines, and is particularly enriched in lung epithelium. Recent studies have shown that increased ACE2 expression is likely to help prevent secondary fibrosis changes following COVID-19 pneumonia.

References:

1. Li Q, et al. (2020). Clin Sci (Lond). 134:2581-2595.
2. Li Y, et al. (2020). Biochem Biophys Res Commun. 526:947-952.
3. Hikmet F, et al. (2020). Mol Syst Biol. 16:e9610.
4. Chaudhry F, et al. (2020). Open Heart. 7:e001424.