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HuR/ELAVL1 Recombinant antibody

HuR/ELAVL1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4L10

Cat no : 82690-2-RR

Synonyms

ELAVL1, Hu antigen R, ELAV-like protein 1, ELAVL 1, ELAV1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HuR/ELAVL1 Recombinant antibody (82690-2-RR)

    WB analysis using 82690-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82690-2-RR (HuR/ELAVL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HCT 116 cells, NCI-H1299 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82690-2-RR targets HuR/ELAVL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HuR/ELAVL1 fusion protein Ag2249
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 30-37 kDa
GenBank Accession NumberBC003376
Gene Symbol HuR/ELAVL1
Gene ID (NCBI) 1994
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

Protocols

Product Specific Protocols
WB protocol for HuR/ELAVL1 antibody 82690-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols