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Histone H1.0 Recombinant antibody, PBS Only

Histone H1.0 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241147F6

Cat no : 83993-4-PBS

Synonyms

H1F0, H1FV, H1-0, H10, H1 histone family, member 0

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse colon tissue using Histone H1.0 Recombinant antibody (83993-4-RR)

    IHC staining of mouse colon using 83993-4-RR (same clone as 83993-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83993-4-RR (Histone H1.0 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83993-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Histone H1.0 Recombinant antibody (83993-4-RR)

    IHC staining of mouse colon using 83993-4-RR (same clone as 83993-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83993-4-RR (Histone H1.0 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83993-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Histone H1.0 Recombinant antibody (83993-4-RR)

    IF Staining of HepG2 using 83993-4-RR (same clone as 83993-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H1.0 antibody (83993-4-RR, Clone: 241147F6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83993-4-PBS in a different storage buffer formulation.

Product Information

The immunogen of 83993-4-PBS is Histone H1.0 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Histone H1.0 fusion protein Ag9982
Full Name H1 histone family, member 0
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC000145
Gene Symbol Histone H1.0
Gene ID (NCBI) 3005
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones.