Histone H1.0 Recombinant antibody, PBS Only

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  IHC staining of mouse colon using 83993-4-RR (same clone as 83993-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83993-4-RR (Histone H1.0 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83993-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 83993-4-RR (same clone as 83993-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83993-4-RR (Histone H1.0 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83993-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83993-4-RR (same clone as 83993-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H1.0 antibody (83993-4-RR, Clone: 241147F6 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83993-4-PBS in a different storage buffer formulation.

Histone H1.0 {ptg:Brand} Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 83993-4-PBS

Clone No.241147F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

H1F0, H1FV, H1-0, H10, H1 histone family, member 0

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Product Information

83993-4-PBS targets Histone H1.0 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Histone H1.0 fusion protein Ag9982
• Full Name: H1 histone family, member 0
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC000145
• Gene Symbol: Histone H1.0
• Gene ID (NCBI): 3005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P07305
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in forming higher order structure in chromatin and maintaining overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones.