ChromoTek Histone-Chromobody® plasmid (eGFP)
DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.
Vector type
Mammalian expression vector
Promoter
Constitutive CMV IE
Reporter
EGFP
Epitope
Histone
Cat no : tcg
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.
Applications | Live cell imaging |
Specificity | Histone H2A-H2B heterodimer, tested in mammalian cells, flies, and yeast |
Vector type | Mammalian expression vector |
Promoter | Constitutive CMV IE |
Reporter | EGFP |
Codon usage | Mammalian |
Selection | Kan/Neo |
Encoded Nanobody/ VHH | Monoclonal anti-H2A-H2B single domain antibody (sdAb) fragment |
Sequence | With the Histone-Chromobody plasmid you receive the sequence information of the alpaca antibody to H2A-H2B heterodimer fused to EGFP and the full vector sequence. |
Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy |
Transfection | ransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
Storage Condition | Shipped at ambient temperature. Store at -20 °C |
Publications
Application | Title |
---|---|
Nat Commun Helicase LSH/Hells regulates kinetochore function, histone H3/Thr3 phosphorylation and centromere transcription during oocyte meiosis. |