ChromoTek Histone-Chromobody® plasmid (eGFP)

DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.

Specificity

Histone

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

EGFP

Publications

1

Cat no : tcg

Synonyms

Chromobody, Histone Chromobody

---

Validation Data Gallery

• 

  Live-cell imaging of Hela cells expressing Histone-Chromobody. Scale bar, 10 µm

• 

  Time-lapse imaging of HeLa cells expressing the Histone-Chromobody: HeLa cells expressing Histone-Chromobody throughout the cell cycle. Interphase chromatin, chromatin condensation and mitotic chromosomes are visible. Scale bar, 10 µm

---

Product Information

DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP.

• Description: The ChromoTek Histone-Chromobody* is a probe to visualize chromatin dynamics in live cells.

  • Easily transfect your cells with the Histone-Chromobody plasmid

  • Histone-Chromobody as marker of chromatin, nucleosomes, and histones

  • Histone-Chromobody does not intercalate in DNA

  • Histone-Chromobody is non-toxic for cells over extended periods of time (up to 3 days)

  *Commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment
• Applications: Non-invasive live cell imaging; Monitors chromatin dynamics; Histone and chromatin as biomarker
• Specificity: Histone H2A-H2B heterodimer, tested in mammalian cells, flies, and yeast
• Reporter: EGFP
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Codon usage: Mammalian
• Selection: Kan/Neo
• Sequence: With the Histone-Chromobody plasmid you receive the sequence information of the alpaca antibody to H2A-H2B heterodimer fused to EGFP and the full vector sequence.
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
	Nat Commun; Helicase LSH/Hells regulates kinetochore function, histone H3/Thr3 phosphorylation and centromere transcription during oocyte meiosis.; Authors - Claudia Baumann