Cobalt Chloride treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82989-4-RR (Hif1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Cobalt Chloride treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82989-4-RR (Hif1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 82989-4-RR
Biolayer interferometry (BLl) kinetic assays of 82989-4-RR against Mouse Hif1a were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Cobalt Chloride treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82989-4-RR (Hif1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 82989-4-RR
Biolayer interferometry (BLl) kinetic assays of 82989-4-RR against Mouse Hif1a were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Hif-1 alpha Recombinant antibody
Catalog Number
82989-4-RR
Citations
-
Dilutions
WB : 1:5000-1:50000
Applications
WB, ELISA
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
