Hif-1 alpha Recombinant antibody

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  WB analysis of NIH/3T3 using 82989-4-RR

  Cobalt Chloride treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82989-4-RR (Hif1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82989-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82989-4-RR against Mouse Hif1a were performed. The affinity constant is below 1 pM.

Hif-1 alpha Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 82989-4-RR

Clone No.230101E8

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Hif1a, 230101E8, Hypoxia inducible factor 1 alpha, HIF1α, HIF1alpha

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Tested Applications

• Positive WB detected in: Cobalt Chloride treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

82989-4-RR targets Hif-1 alpha in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Hif-1 alpha fusion protein Ag33620
• Full Name: hypoxia inducible factor 1, alpha subunit
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_001313919
• Gene Symbol: Hif1a
• Gene ID (NCBI): 15251
• RRID: AB_3670737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q61221
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Carbohydr Polym; Carboxymethyl chitosan and sodium alginate oxide pH-sensitive dual-release hydrogel for diabetes wound healing: The combination of astilbin liposomes and diclofenac sodium; Authors - Xiaoyu Wu