HYAL3 Polyclonal antibody

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  WB analysis of human brain using 19625-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19625-1-AP (HYAL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19625-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19625-1-AP (HYAL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19625-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 19625-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 19625-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 19625-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 19625-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19625-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19625-1-AP (HYAL3 antibody) at dilution of 1:100 (under 40x lens).

HYAL3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19625-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Hyal 3, HYAL3, Hyaluronidase 3, hyaluronoglucosaminidase 3, LUCA 3, LUCA14, LUCA3, Lung carcinoma protein 3, Minna14

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Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells
• Positive IHC detected in: human testis tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

19625-1-AP targets HYAL3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HYAL3 fusion protein Ag8611
• Full Name: hyaluronoglucosaminidase 3
• Calculated Molecular Weight: 417aa,47 kDa; 463aa,52 kDa
• Observed Molecular Weight: 47 kDa, 55 kDa
• GenBank Accession Number: BC005896
• Gene Symbol: HYAL3
• Gene ID (NCBI): 8372
• RRID: AB_10666437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43820
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	J Gastroenterol Hepatol; The suppressive role of HYAL1 and HYAL2 in the metastasis of colorectal cancer.; Authors - Zhiming Jin