HYAL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HYAL2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 51148-1-AP

Synonyms

Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2

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Validation Data Gallery

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  WB analysis of mouse heart using 51148-1-AP

  mouse heart tissue and mouse liver tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 51148-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 51148-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 51148-1-AP (HYAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 51148-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 51148-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51148-1-AP (HYAL2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 51148-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 51148-1-AP (HYAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, human lung tissue, mouse liver tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

51148-1-AP targets HYAL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HYAL2 fusion protein Ag0582
• Full Name: hyaluronoglucosaminidase 2
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC000692
• Gene Symbol: HYAL2
• Gene ID (NCBI): 8692
• RRID: AB_2123391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).

Publications

Species	Application	Title
human	WB,IHC	Dev Cell; High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.; Authors - Takuya Ooki ; KD Validated

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