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HYAL1 Polyclonal antibody, PBS Only

HYAL1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25179-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HYAL1 Polyclonal antibody (25179-1-AP)

    WB analysis using 25179-1-AP (same clone as 25179-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25179-1-AP (HYAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25179-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HYAL1 Polyclonal antibody (25179-1-AP)

    IHC staining of mouse liver using 25179-1-AP (same clone as 25179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25179-1-AP (HYAL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25179-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HYAL1 Polyclonal antibody (25179-1-AP)

    IHC staining of mouse liver using 25179-1-AP (same clone as 25179-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25179-1-AP (HYAL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25179-1-PBS in a different storage buffer formulation.

Product Information

25179-1-PBS targets HYAL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HYAL1 fusion protein Ag17943
Full Name hyaluronoglucosaminidase 1
Calculated Molecular Weight 435 aa, 48 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC035695
Gene Symbol HYAL1
Gene ID (NCBI) 3373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL-1 is present in many tissues and is predominantly found in the plasma and urine (PMID: 16600643). In addition to its function in normal cellular hyaluronan turnover, human HYAL-1 is implicated in cancer proliferation, angiogenesis, and inflammatory diseases (PMID: 17503783).