Huntingtin Polyclonal antibody

Huntingtin Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19957-1-AP

Synonyms

HTT, IT15, Huntington disease protein, Huntingtin, myristoylated N-terminal fragment, HD protein

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Validation Data Gallery

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  IHC staining of mouse brain using 19957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19957-1-AP (Huntingtin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 19957-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using Huntingtin antibody (19957-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of SH-SY5Y using 19957-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Huntingtin antibody (19957-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells, SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19957-1-AP targets Huntingtin in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: huntingtin
• Calculated Molecular Weight: 348 kDa
• GenBank Accession Number: NM_002111
• Gene Symbol: Huntingtin
• Gene ID (NCBI): 3064
• RRID: AB_2878629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTT, also named as HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. The antibody recognizes the C-term of HTT.