HTRA2 Recombinant antibody, PBS Only

HTRA2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240496D1

Cat no : 83492-7-PBS

Synonyms

PARK13, Omi stress-regulated endoprotease, OMI, HtrA serine peptidase 2, EC:3.4.21.108

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Validation Data Gallery

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  WB analysis using 83492-7-RR (same clone as 83492-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83492-7-RR (HTRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83492-7-RR (same clone as 83492-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HTRA2 antibody (83492-7-RR, Clone: 240496D1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83492-7-RR (same clone as 83492-7-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HTRA2 Recombinant antibody (83492-7-RR, Clone:240496D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83492-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83492-7-RR against Human HTRA2 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83492-7-PBS is HTRA2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HTRA2 fusion protein Ag8455
• Full Name: HtrA serine peptidase 2
• Calculated Molecular Weight: 49 kDa
• GenBank Accession Number: BC000096
• Gene Symbol: HTRA2
• Gene ID (NCBI): 27429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.