HTRA2 Recombinant antibody

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  IF Staining of HepG2 using 83492-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HTRA2 antibody (83492-6-RR, Clone: 240496E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HeLa using 83492-6-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HTRA2 Recombinant antibody (83492-6-RR, Clone:240496E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83492-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83492-6-RR against Human HTRA2 were performed. The affinity constant is 9.83 nM.

HTRA2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83492-6-RR

Clone No.240496E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

PARK13, Omi stress-regulated endoprotease, OMI, HtrA serine peptidase 2, EC:3.4.21.108

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83492-6-RR targets HTRA2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HTRA2 fusion protein Ag8455
• Full Name: HtrA serine peptidase 2
• Calculated Molecular Weight: 49 kDa
• GenBank Accession Number: BC000096
• Gene Symbol: HTRA2
• Gene ID (NCBI): 27429
• RRID: AB_3671118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43464
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.