HTR7 Polyclonal antibody

HTR7 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 29238-1-AP

Synonyms

5-HT-7, 5-HT7, 5 hydroxytryptamine receptor 7, 5 HT7, 5 HT X

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Validation Data Gallery

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  IHC staining of mouse cerebellum using 29238-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29238-1-AP (HTR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29238-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29238-1-AP (HTR7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29238-1-AP targets HTR7 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HTR7 fusion protein Ag30856
• Full Name: 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled)
• Calculated Molecular Weight: 445 aa, 49 kDa
• GenBank Accession Number: BC047526
• Gene Symbol: HTR7
• Gene ID (NCBI): 3363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 5-hydroxytryptamine receptor 7 (HTR7), also known as 5-HT7, is a G protein-coupled receptor that is activated by the neurotransmitter serotonin (5-HT). It is involved in the G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger and chemical synaptic transmission. It is expressed in several structures, including adipose tissue, the alimentary system, the brain, the immune system, and the reproductive system.