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HTR5A Polyclonal antibody

HTR5A Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 28221-1-AP

Synonyms

5 HT 5, 5 HT 5A, 5 HT5A, HTR5A, Serotonin receptor 5A

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using HTR5A Polyclonal antibody (28221-1-AP)

    IHC staining of mouse brain using 28221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28221-1-AP (HTR5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HTR5A Polyclonal antibody (28221-1-AP)

    IHC staining of mouse brain using 28221-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28221-1-AP (HTR5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28221-1-AP targets HTR5A in IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HTR5A fusion protein Ag28177
Full Name 5-hydroxytryptamine (serotonin) receptor 5A
Calculated Molecular Weight 357 aa, 48 kDa
GenBank Accession NumberBC112021
Gene Symbol HTR5A
Gene ID (NCBI) 3361
RRIDAB_2918151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for HTR5A antibody 28221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols