HTR1B Polyclonal antibody

HTR1B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22189-1-AP

Synonyms

5 HT 1B, 5 HT 1D beta, 5 HT1B, 5 HT1DB, HTR1B, HTR1D2, HTR1DB, S12, Serotonin 1D beta receptor, Serotonin receptor 1B

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Validation Data Gallery

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  WB analysis of human placenta using 22189-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 22189-1-AP (HTR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 22189-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22189-1-AP (HTR1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 22189-1-AP is HTR1B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HTR1B fusion protein Ag16753
• Full Name: 5-hydroxytryptamine (serotonin) receptor 1B
• Calculated Molecular Weight: 390 aa, 44 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: BC096207
• Gene Symbol: HTR1B
• Gene ID (NCBI): 3351
• RRID: AB_2879020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTR1B, also known as 5-HT1B, 5-HT1DB, HTR1D2, HTR1DB, and S12, belongs to the G-protein coupled receptor 1 family. HTR1B is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of HTR1B is mediated by G proteins that inhibit adenylate cyclase activity.

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse.; Authors - Huiduo Guo
human	WB,IHC	Heliyon; HTR1B regulates mitochondrial homeostasis and mitophagy by activating the ERK/ MAPK signalling pathway during human embryonic arrest; Authors - Si-Min Ding