HSPBAP1 Polyclonal antibody

HSPBAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27771-1-AP

Synonyms

HSPB1 associated protein 1, HSPBAP1, PASS1

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Validation Data Gallery

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  WB analysis of PC-3 using 27771-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27771-1-AP (HSPBAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 27771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27771-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27771-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27771-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27771-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27771-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27771-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27771-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: mouse kidney tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27771-1-AP targets HSPBAP1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSPBAP1 fusion protein Ag26808
• Full Name: HSPB (heat shock 27kDa) associated protein 1
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC011897
• Gene Symbol: HSPBAP1
• Gene ID (NCBI): 79663
• RRID: AB_2880968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPBAP1, also named as Protein associated with small stress protein 1, is a widely expressed 488 amino acid protein. HSPBAP1 may play a role in regulating stress response in the living cell(PMID: 11978969). A chromosomal aberration involving HSPBAP1 has been found in a family with renal carcinoma (PMID:12939738). HSPBAP1 has multiple isoforms with MW 55, 50, 32 and 25 kDa.