HSPB8 Polyclonal antibody

HSPB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 15287-1-AP

Synonyms

E2-induced gene 1 protein, E2IG1, DHMN2, CRYAC, CMT2L

---

Validation Data Gallery

• 

  WB analysis using 15287-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 15287-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15287-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15287-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 15287-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: Neuro-2a cells, SGC-7901 cells, mouse brain tissue, HEK-293 cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human heart tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Product Information

15287-1-AP targets HSPB8 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSPB8 fusion protein Ag7456
• Full Name: heat shock 22kDa protein 8
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20-22 kDa
• GenBank Accession Number: BC002673
• Gene Symbol: HSPB8
• Gene ID (NCBI): 26353
• RRID: AB_2248640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IF	Nat Commun; Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.; Authors - Thomas G Martin
mouse	IHC	J Neurosci; The α crystallin domain of small heat shock protein b8 (Hspb8) acts as survival and differentiation factor in adult hippocampal neurogenesis.; Authors - Ramírez-Rodríguez Gerardo G
human	WB	J Am Heart Assoc; Reduction of Filamin C Results in Altered Proteostasis, Cardiomyopathy, and Arrhythmias; Authors - Joyce C Ohiri
mouse	WB	J Cell Biochem; Pharmacological inhibition of BAG3-HSP70 with the proposed cancer therapeutic JG-98 is toxic for cardiomyocytes.; Authors - Thomas G Martin
mouse,human	CoIP	Genes (Basel); BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation.; Authors - Hongtao Diao
human	WB	Cell Rep; Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR.; Authors - Dawid Krokowski