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GRP75 Polyclonal antibody

GRP75 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 14887-1-AP


CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74

Tested Applications

Positive WB detected inHT-29 cells, A549 cells, mouse testis tissue, Jurkat cells, NIH/3T3 cells, mouse liver
Positive IP detected inmouse brain tissue, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14887-1-AP targets GRP75 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP75 fusion protein Ag6673
Full Name heat shock 70kDa protein 9 (mortalin)
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 74-75 kDa
GenBank Accession NumberBC000478
Gene Symbol GRP75
Gene ID (NCBI) 3313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)


Product Specific Protocols
WB protocol for GRP75 antibody 14887-1-APDownload protocol
IHC protocol for GRP75 antibody 14887-1-APDownload protocol
IF protocol for GRP75 antibody 14887-1-APDownload protocol
IP protocol for GRP75 antibody 14887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Authors - Ricarda Richter-Dennerlein

Nat Commun

LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.

Authors - Chun-Shik Shin

EMBO Mol Med

Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.

Authors - Masayuki Ide

J Cell Biol

Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.

Authors - Anne Korwitz

Cell Death Dis

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats

Authors - Yajie Wang

Cell Mol Gastroenterol Hepatol

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Authors - Dayan Sun


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Christina (Verified Customer) (11-14-2023)

I used this antibody for HeLa extracts in Western Blot and worked perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HeLa cells

Ning (Verified Customer) (02-27-2023)

Specifically probe endogenous GRP75 with a right MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
GRP75 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:14887-1-AP)

Staci (Verified Customer) (02-02-2023)

Antibody works very well with GRP75. Very little non-specific binding on immunoblot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Mouse Islet protein lysates