HSPA6 Monoclonal antibody

HSPA6 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3D12D7

Cat no : 68257-1-Ig

Synonyms

Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, HSP70B, HSPA6

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Validation Data Gallery

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  WB analysis using 68257-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68257-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 68257-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 68257-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using HSPA6 antibody (68257-1-Ig, Clone: 3D12D7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

68257-1-Ig targets HSPA6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSPA6 fusion protein Ag4716
• Full Name: heat shock 70kDa protein 6 (HSP70B')
• Calculated Molecular Weight: 643 aa, 71 kDa
• Observed Molecular Weight: 68-71 kDa
• GenBank Accession Number: BC035665
• Gene Symbol: HSPA6
• Gene ID (NCBI): 3310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)

Publications

Species	Application	Title
human	WB,IHC	Biochim Biophys Acta Mol Cell Res; PP4R1 accelerates the malignant progression of NSCLC via up-regulating HSPA6 expression and HSPA6-mediated ER stress; Authors - Xunxia Zhu