HSPA2 Recombinant antibody, PBS Only (Capture)

HSPA2 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240421A2

Cat no : 83466-1-PBS

Synonyms

HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2, 240421A2

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Validation Data Gallery

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  WB analysis using 83466-1-RR (same clone as 83466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83466-1-RR (HSPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83466-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00465-1

  Cytometric bead array standard curve of MP00465-1, HSPA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83466-1-PBS. Detection antibody: 83466-3-PBS. Standard: Ag3539. Range: 0.625-80 ng/mL.

• 

  Cytometric bead array standard curve of MP00465-2

  Cytometric bead array standard curve of MP00465-2, HSPA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83466-1-PBS. Detection antibody: 83466-2-PBS. Standard: Ag3539. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83466-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 83466-1-RR against Human HSPA2 were performed. The affinity constant is 1.86 nM.

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Product Information

83466-1-PBS targets HSPA2 as part of a matched antibody pair:

MP00465-1: 83466-1-PBS capture and 83466-3-PBS detection (validated in Cytometric bead array)

MP00465-2: 83466-1-PBS capture and 83466-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HSPA2 fusion protein Ag3539
• Full Name: heat shock 70kDa protein 2
• Calculated Molecular Weight: 639 aa, 70 kDa
• GenBank Accession Number: BC036107
• Gene Symbol: HSPA2
• Gene ID (NCBI): 3306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.