GRP94 Monoclonal antibody

GRP94 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1H10B7

Cat no : 60012-1-Ig

Synonyms

ECGP, GP96, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

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Validation Data Gallery

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  WB analysis of human heart using 60012-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 60012-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60012-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60012-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60012-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60012-1-Ig (GRP94 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 60012-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 60012-1-Ig

  human lung tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60012-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60012-1-Ig

  Immunofluorescent analysis of Hela cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  IF Staining of MCF-7 using 60012-1-Ig

  Immunofluorescent analysis of MCF-7 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

• 

  IF Staining of HepG2 using 60012-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Tested Applications

• Positive WB detected in: Human heart, human heart tissue, human liver tissue, human lung tissue, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Hela cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• CoIP: See 1 publications below

Product Information

60012-1-Ig targets GRP94 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRP94 fusion protein Ag1439
• Full Name: heat shock protein 90kDa beta (Grp94), member 1
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC009195
• Gene Symbol: GRP94
• Gene ID (NCBI): 7184
• RRID: AB_2119056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Publications

Species	Application	Title
human	WB	Autophagy; Influenza M2 protein regulates MAVS-mediated signaling pathway through interacting with MAVS and increasing ROS production.; Authors - Ruifang Wang
human	WB	Front Cell Dev Biol; Extracellular Vesicles Carry lncRNA SNHG16 to Promote Metastasis of Breast Cancer Cells via the miR-892b/PPAPDC1A Axis.; Authors - Wenfei Xia
rat	CoIP	Neurochem Res; Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1.; Authors - Jiajia Chen
human	WB	J Adv Res; Development of a novel thymidylate synthase (TS) inhibitor capable of up-regulating P53 expression and inhibiting angiogenesis in NSCLC.; Authors - Xin-Yang Li
mouse	WB	Cell Rep; Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.; Authors - Fangfang Sun
		Front Physiol; Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study.; Authors - Raul Chavez-Valdez