Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60012-2-Ig targets GRP94 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1439 Product name: Recombinant human GRP94 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC009195 Sequence: MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAARRR Predict reactive species |
| Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Calculated Molecular Weight | 96 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC009195 |
| Gene Symbol | GRP94 |
| Gene ID (NCBI) | 7184 |
| RRID | AB_2881351 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P14625 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| IF protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| IHC protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| WB protocol for GRP94 antibody 60012-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
GRP94 Monoclonal antibody (4G7C7), catalog number 60012-2-Ig, has 12 citations. These appear in journals including J Extracell Vesicles, J Control Release, Phytomedicine, J Transl Med, Haematologica, Int J Mol Sci, Front Pharmacol, Virol Sin, Bone, J Clin Med, Biomol Biomed, and Anticancer Drugs. Research fields span extracellular vesicle biology, diabetic nephropathy, Parkinson's disease, uveitis, leukemia, multiple myeloma, NSCLC, viral infection, bone biology, and colorectal cancer.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles CKAP4 in Extracellular Vesicle-Derived From Podocyte Serves as a Non-Invasive Diagnostic Biomarker for Diabetic Nephropathy and Promotes Vascular Calcification. | ||
J Control Release Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility | ||
Phytomedicine Phillyrin promotes autophagosome formation in A53T-αSyn-induced Parkinson's disease model via modulation of REEP1 | ||
J Transl Med Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration | ||
Haematologica DNAJC10 maintains survival and self-renewal of leukemia stem cells through PERK branch of the unfolded protein response | ||
Int J Mol Sci Size-Exclusion Chromatography: A Path to Higher Yield and Reproducibility Compared to Sucrose Cushion Ultracentrifugation for Extracellular Vesicle Isolation in Multiple Myeloma |
Reviews
What customers say
The single review for this product is highly positive. A user rated it 5 stars and reported that it worked well at a 1:2000 dilution for Western Blot on MSCs (mesenchymal stem cells). The review was posted in August 2024. Overall, the feedback suggests reliable performance under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Udesh (Verified Customer) (08-19-2024) | Worked well at 1:2000
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