HSP70 Monoclonal antibody, PBS Only

HSP70 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, yeast

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E7E5

Cat no : 66183-1-PBS

Synonyms

HSPA1A, HSP72, HSP70.1, Heat shock protein family A member 1A, heat shock 70kDa protein 1A

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Validation Data Gallery

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  WB analysis using 66183-1-Ig (same clone as 66183-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

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  WB analysis of Recombinant protein using 66183-1-Ig (same clone as 66183-1-PBS)

  6*His tagged-recombinant HSP70 (291-641 aa) and HSC70 (346-646 aa) proteins were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 antibody), 66442-1-Ig (HSC70 antibody), and 66005-1-Ig (His antibody), respectively, at dilution of 1:50000, incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66183-1-Ig (same clone as 66183-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66183-1-Ig (same clone as 66183-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66183-1-Ig (same clone as 66183-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

• 

  WB analysis of whole yeast using 66183-1-Ig (same clone as 66183-1-PBS)

  whole yeast cells were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66183-1-Ig (same clone as 66183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66183-1-Ig (HSP70 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66183-1-Ig (same clone as 66183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66183-1-Ig (HSP70 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66183-1-Ig (same clone as 66183-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HEK-293 cells using 66183-1-Ig(HSP70 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66183-1-Ig (same clone as 66183-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human HSP70 (66183-1-Ig, Clone:4E7E5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

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Product Information

66183-1-PBS targets HSP70 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, yeast samples.

• Tested Reactivity: human, mouse, rat, yeast
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP70 fusion protein Ag19111
• Full Name: heat shock 70kDa protein 1A
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC009322
• Gene Symbol: HSP70
• Gene ID (NCBI): 3303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)