HSP47/SERPINH1 Recombinant antibody

HSP47/SERPINH1 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240752F6

Cat no : 83689-3-RR

Synonyms

HSP47, SERPINH1, CBP2, CBP1, AsTP3

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Validation Data Gallery

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  WB analysis using 83689-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovarian cancer using 83689-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83689-3-RR (HSP47/SERPINH1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 83689-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HSP47/SERPINH1 Recombinant antibody (83689-3-RR, Clone:240752F6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83689-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83689-3-RR against Human HSP47/SERPINH1 were performed. The affinity constant is 12.9 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, HT-1080 cells, SK-N-SH cells, NIH/3T3 cells
• Positive IHC detected in: human ovarian cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83689-3-RR is HSP47/SERPINH1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Calculated Molecular Weight: 46kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: NM_001235.5
• Gene Symbol: HSP47/SERPINH1
• Gene ID (NCBI): 871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.