HSF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 51034-1-AP

Synonyms

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

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Validation Data Gallery

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  WB analysis using 51034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 51034-1-AP

  WB result of HSF1 antibody (51034-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSF1 transfected HeLa cells.

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  WB analysis using 51034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 51034-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 51034-1-AP

  IP result of anti-HSF1 (IP:51034-1-AP, 4ug; Detection:51034-1-AP 1:8000) with Jurkat cells lysate 1560 ug.

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  IHC staining of human lung cancer using 51034-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51034-1-AP (HSF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 51034-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51034-1-AP (HSF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 51034-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51034-1-AP (HSF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 51034-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51034-1-AP (HSF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 51034-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using HSF1 antibody (51034-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, A431 cells, HepG2 cells, K-562 cells, NIH/3T3 cells, HEK-293T cells, C6 cells, mouse ovary tissue, mouse testis tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human lung cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

51034-1-AP targets HSF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSF1 fusion protein Ag0739
• Full Name: heat shock transcription factor 1
• Calculated Molecular Weight: 529 aa, 57 kDa
• Observed Molecular Weight: 68-80 kDa
• GenBank Accession Number: BC014638
• Gene Symbol: HSF1
• Gene ID (NCBI): 3297
• RRID: AB_2120269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

Publications

Species	Application	Title
mouse	WB	Science; Z-DNA binding protein 1 promotes heatstroke-induced cell death.; Authors - Fangfang Yuan
mouse	WB	Nat Commun; Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy; Authors - Donghai Cui
mouse	WB	Food Funct; Protective effects of L-theanine and dihydromyricetin on reproductive function in male mice under heat stress; Authors - Difei Yang
rat		Int J Pharm; Effective myocardial infarction treatment by targeted accumulation of Sulforaphane using porous magnetic silica nanoparticles; Authors - Jian Zhang
human	WB	Am J Physiol Cell Physiol; Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1; Authors - Jie Zhao
mouse	WB	J Gerontol A Biol Sci Med Sci; Multifactorial Attenuation of the Murine Heat Shock Response With Age.; Authors - Donald A Jurivich