HSF1 Polyclonal antibody, PBS Only

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  WB analysis of K-562 using 16107-1-AP (same clone as 16107-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16107-1-AP (same clone as 16107-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 16107-1-AP (same clone as 16107-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 16107-1-AP (same clone as 16107-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 16107-1-AP (same clone as 16107-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 16107-1-AP (same clone as 16107-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

HSF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16107-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

16107-1-PBS targets HSF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSF1 fusion protein Ag9023
• Full Name: heat shock transcription factor 1
• Calculated Molecular Weight: 529 aa, 57 kDa
• Observed Molecular Weight: 68-80 kDa
• GenBank Accession Number: BC014638
• Gene Symbol: HSF1
• Gene ID (NCBI): 3297
• RRID: AB_11182610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q00613
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).