HSDL2 Monoclonal antibody

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  WB analysis using 68414-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68414-1-Ig (HSDL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68414-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSDL2 antibody (68414-1-Ig, Clone: 3D11G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

HSDL2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68414-1-Ig

Clone No.3D11G1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

C9orf99, FLJ25855, HSDL2, SDR13C1

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, pig heart tissue, rabbit heart tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68414-1-Ig targets HSDL2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit samples.

• Tested Reactivity: Human, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSDL2 fusion protein Ag8314
• Full Name: hydroxysteroid dehydrogenase like 2
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC004331
• Gene Symbol: HSDL2
• Gene ID (NCBI): 84263
• RRID: AB_3085130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6YN16
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)