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  • KD/KO Validated

HSDL2 Polyclonal antibody

HSDL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15631-1-AP

Synonyms

Short chain dehydrogenase/reductase family 13C member 1, SDR13C1, Hydroxysteroid dehydrogenase-like protein 2, EC:1.-.-.-, C9orf99



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15631-1-AP targets HSDL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSDL2 fusion protein Ag7987
Full Name hydroxysteroid dehydrogenase like 2
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC004331
Gene Symbol HSDL2
Gene ID (NCBI) 84263
RRIDAB_2120238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)

Protocols

Product Specific Protocols
WB protocol for HSDL2 antibody 15631-1-APDownload protocol
IHC protocol for HSDL2 antibody 15631-1-APDownload protocol
IF protocol for HSDL2 antibody 15631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics

Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells.

Authors - Songlin Zeng
humanIF

Cancer Manag Res

Ectopic expression of HSDL2 is related to cell proliferation and prognosis in breast cancer.

Authors - Bing Dong
humanIHC,WB

Onco Targets Ther

HSDL2 Acts as a Promoter in Pancreatic Cancer by Regulating Cell Proliferation and Lipid Metabolism.

Authors - Anna Han
  • KD Validated
humanWB,FC

Chin Med

Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression.

Authors - Wen-Bei Liu
  • KD Validated
humanIHC

Nan Fang Yi Ke Da Xue Xue Bao

[HSDL2 overexpression promotes rectal cancer progression by regulating cancer cell cycle and promoting cell proliferation]

Authors - Y Cheng
  • KD Validated
mouse,humanWB,IF

Sci Adv

HSDL2 links nutritional cues to bile acid and cholesterol homeostasis

Authors - Nolwenn Samson