HSD3B2 Monoclonal antibody, PBS Only

HSD3B2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E7B4

Cat no : 67572-1-PBS

Synonyms

3-beta-HSD II, 3-beta-HSD adrenal and gonadal type, 3b HSD, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2

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Validation Data Gallery

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  WB analysis using 67572-1-Ig (same clone as 67572-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67572-1-Ig (same clone as 67572-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 67572-1-Ig (same clone as 67572-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human adrenal gland using 67572-1-Ig (same clone as 67572-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig adrenal gland using 67572-1-Ig (same clone as 67572-1-PBS)

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67572-1-Ig (same clone as 67572-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HSD3B2 antibody (67572-1-Ig, Clone: 2E7B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

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Product Information

67572-1-PBS targets HSD3B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSD3B2 fusion protein Ag4291
• Full Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC038419
• Gene Symbol: HSD3B2
• Gene ID (NCBI): 3284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in placenta and skin(PMID:1682237). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).