HSD3B2 Polyclonal antibody

HSD3B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15516-1-AP

Synonyms

3b HSD, HSD3B2, HSD3β2, HSDB, HSDB3, HSDB3B, SDR11E2

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Validation Data Gallery

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  WB analysis of mouse testis using 15516-1-AP

  mouse testis tissue was subjected to SDS PAGE followed by western blot with 15516-1-AP (HSD3B2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 15516-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15516-1-AP (HSD3B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 15516-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15516-1-AP (HSD3B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

15516-1-AP targets HSD3B2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD3B2 fusion protein Ag7782
• Full Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC038419
• Gene Symbol: HSD3B2
• Gene ID (NCBI): 3284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in placenta and skin (PMID:1682237). HSD3B2 plays a crucial role in steroid hormone biosynthesis and is thus of particular interest in hormone dependent tumors such as prostate cancer. Compared with normal tissue cytoplasmic HSD3B2 staining was stronger in prostate cancers (PMID: 29803408). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).

Publications

Species	Application	Title
mouse	WB	J Hazard Mater; Tributyltin chloride (TBTCL) induces cell injury via dysregulation of endoplasmic reticulum stress and autophagy in Leydig cells; Authors - Pengchen Chen
human	WB	Oncotarget; Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase.; Authors - Liping Wen
rat	WB, IHC	Endocrinology; A dietary medium-chain fatty acid, decanoic acid, inhibits recruitment of Nur77 to the HSD3B2 promoter in vitro, and reverses endocrine and metabolic abnormalities in a rat model of polycystic ovary syndrome.; Authors - Bao Hui Lee
mouse	IF	Front Physiol; Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation.; Authors - Guillaume Richer
mouse	IHC	Reprod Biomed Online; Brain-derived neurotrophic factor expression in granulosa lutein cells.; Authors - Dominguez Miguel A MA
human	IHC	Int J Clin Exp Pathol; Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile.; Authors - Anli Tong