HSD17B2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSD17B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

33

Cat no : 10978-1-AP

Synonyms

17 beta HSD 2, 17βHSD2, 20 alpha HSD, E2DH, EDH17B2, HSD17, HSD17B2, SDR9C2

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Validation Data Gallery

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  WB analysis of HepG2 using 10978-1-AP

  WB result of HSD17B2 antibody (10978-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B2 transfected HepG2 cells.

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  WB analysis using 10978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10978-1-AP (HSD17B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10978-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10978-1-AP (HSD17B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10978-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10978-1-AP (HSD17B2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 10978-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10978-1-AP (HSD17B2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 10978-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10978-1-AP (HSD17B2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, human placenta tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IHC: See 21 publications below

Product Information

10978-1-AP targets HSD17B2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD17B2 fusion protein Ag1411
• Full Name: hydroxysteroid (17-beta) dehydrogenase 2
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC009581
• Gene Symbol: HSD17B2
• Gene ID (NCBI): 3294
• RRID: AB_2279863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSD17B2(Estradiol 17-beta-dehydrogenase 2) is also named as 17-beta-HSD 2,20-alpha-HSD,EDH17B2 and belongs to the short-chain dehydrogenases/reductases (SDR) family.Based on the pivotal role of HSD17B2 gene product in the estrogen metabolism pathway, it may be considered as a putative candidate gene that could possibly explain a fraction of the remaining familial breast cancer risk and it is unlikely to play a role as a high penetrance gene in breast cancer predisposition(PMID:18372405).

Publications

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
human	IHC	J Clin Endocrinol Metab; MUC1 as a discriminator between endometrium from fertile and infertile patients with PCOS and endometriosis.; Authors - Margarit L L
human	IHC	Br J Cancer; In breast cancer subtypes steroid sulfatase (STS) is associated with less aggressive tumour characteristics.; Authors - Keely M McNamara
human	WB,IHC	Fertil Steril; Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.; Authors - Miao Sun
human	WB,IHC	Endocr Relat Cancer; PMID: 20228125; Authors - Takagi Kiyoshi K
rat	WB	Ecotoxicol Environ Saf; Cypermethrin triggers YY1-mediated testosterone biosynthesis suppression.; Authors - Changjiang Liu