HS2ST1 Polyclonal antibody

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  WB analysis of human liver using 12453-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12453-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12453-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12453-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12453-1-AP (HS2ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 12453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12453-1-AP (HS2ST1 antibody) at dilution of 1:50 (under 10x lens).

HS2ST1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12453-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

2 O sulfotransferase, 2OST, HS2ST, HS2ST1, KIAA0448

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Tested Applications

• Positive WB detected in: human liver tissue, human brain tissue, human heart tissue, PC-3 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

12453-1-AP targets HS2ST1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HS2ST1 fusion protein Ag3111
• Full Name: heparan sulfate 2-O-sulfotransferase 1
• Calculated Molecular Weight: 229 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC025990
• Gene Symbol: HS2ST1
• Gene ID (NCBI): 9653
• RRID: AB_2119312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7LGA3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Medicine (Baltimore); Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3; Authors - Guozhi Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bettina (Verified Customer) (08-05-2022)

Weak detection

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human pancreatic cancer cell line

HS2ST1 Antibody Western Blot validation (1:1000 dilution) in Human pancreatic cancer cell line (Cat no:12453-1-AP)