HRP-conjugated Goat Anti-Rabbit IgG(H+L)

Applications

ELISA, WB, Dot blot

Conjugate

HRP

Host

Goat

Reactivity

Rabbit

Publications

10478

Reviews (7)

Cat no : SA00001-2

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Validation Data Gallery

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  IP Result of anti-alpha Tubulin (IP: 66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug. SA00001-2 (HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) ) as the secondary antibody.

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Product Information

• Concentration: 0.2 mg/mL
• Applications: ELISA, WB, Dot blot
• Host Species: Goat
• Species Reactivity: Rabbit
• Physical State: Liquid
• Suggested Dilution Range: 1:2000-1:10,000 for western blotting with ECL substrates; 1:1000-1:20,000 for ELISA and Western blotting with chromogenic substrates
• Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Storage Buffer: 0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 3 mg/ml BSA, pH 7.6.
• Storage Instructions: Store at -20°C. Stable for one year after shipment.
• Stabilizer: 3 mg/ml BSA
• Clonality: Polyclonal
• RRID: AB_2722564
• Note: This product is for research use only,not for diagnostic or therapeutic use.

Publications

Title
Nature; Structural basis for FGF hormone signalling; Authors - Lingfeng Chen
Cell; Red blood cells undergo lytic programmed cell death involving NLRP3; Authors - Yaozhen Chen
Nat Biotechnol; Generation of a live attenuated influenza A vaccine by proteolysis targeting.; Authors - Longlong Si
Nat Methods; Metabolomic profiling of single enlarged lysosomes.; Authors - Hongying Zhu
Nat Immunol; A critical role of N4-acetylation of cytidine in mRNA by NAT10 in T cell expansion and antiviral immunity; Authors - Lu Sun
Cell Host Microbe; Bifidobacterium longum promotes postoperative liver function recovery in patients with hepatocellular carcinoma; Authors - Jingjing Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paula (Verified Customer) (02-06-2025)

Secondary ab works 1:1000-1:4000 depending on the primary ab. Overall, good quality.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human Bone Marrow Mesenchymal Stem Cells

FH

Macarena (Verified Customer) (10-07-2022)

good results when we are running out of our secondaries of choice

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: cell lines

FH

SONIA (Verified Customer) (06-13-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Protein samples from tumor cell lines

FH

Brogan (Verified Customer) (02-22-2022)

Used for an ELISA against a custom made polyclonal antibody and worked brilliantly. Will definitely be ordering this secondary antibody again.

• Applications: Other

FH

Arturo (Verified Customer) (02-17-2022)

Amazing product! I manage to reproduce the same results using the sample now that I was running out of my second antibody for the Western Blots. Now I can take care of the rest of my experiments and finish them for my PhD project.

• Applications: Western Blot

FH

Paolo (Verified Customer) (03-05-2020)

it works perfectly in ELISA experiments

• Applications: Other,

FH

David (Verified Customer) (06-19-2019)

I use this or an anti-mouse HRP conjugate about everyday, I bought this one after trying the mouse version that was previously here in the lab. I like it very much. Most of the time I get excellent and sharp results. The times I don't I think it's more the experiment than your product.

• Applications: Western Blot,