HRAS-Specific Polyclonal antibody

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  WB analysis using 18295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18295-1-AP

  Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18295-1-AP

  WB result of HRAS-Specific antibody (18295-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRAS-Specific transfected HEK-293 cells.

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  WB analysis of HEK-293 using 18295-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18295-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18295-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 18295-1-AP

  IP result of anti-HRAS-Specific (IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with mouse brain tissue lysate 1840 ug.

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  IP experiment of HEK-293 using 18295-1-AP

  IP result of anti-HRAS-Specific (IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with HEK-293 cells lysate 1480 ug.

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  IHC staining of human colon cancer using 18295-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18295-1-AP (HRAS-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IF Staining of MCF-7 using 18295-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HRAS-Specific antibody (18295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 18295-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HRAS-Specific (18295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

HRAS-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18295-1-AP

Publications(62)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

GTPase HRas, H RASIDX, HRAS, HRAS1, EC:3.6.5.2

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Tested Applications

• Positive WB detected in: HEK-293 cells, SH-SY5Y cells, PC-3 cells, C6 cells, mouse kidney tissue, rat tissue, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue, HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 54 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 3 publications below

Product Information

18295-1-AP targets HRAS-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-Ha-ras Harvey rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC006499
• Gene Symbol: HRAS
• Gene ID (NCBI): 3265
• RRID: AB_2121046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01112
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS.; Authors - Sarah J Ross
human	WB	Sci Transl Med; The ERBB network facilitates KRAS-driven lung tumorigenesis.; Authors - Björn Kruspig
human	IP	Mol Cell; KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis; Authors - Hiroshi Senoo
human	WB	J Exp Med; Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition; Authors - Wei Wei
human	IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
human	WB	Nat Commun; A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS.; Authors - Nicolas Bery

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Benjamin (Verified Customer) (01-10-2020)

Identified HRAS around 20kDa mark on western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK