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HRAS-Specific Polyclonal antibody
HRAS-Specific Polyclonal Antibody for FC (Intra), IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 18295-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, PC-3 cells, SH-SY5Y cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat tissue |
Positive IP detected in | mouse brain tissue |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 42 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
Product Information
18295-1-AP targets HRAS-Specific in WB, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | v-Ha-ras Harvey rat sarcoma viral oncogene homolog |
Calculated molecular weight | 21 kDa |
Observed molecular weight | 21 kDa |
GenBank accession number | BC006499 |
Gene symbol | HRAS |
Gene ID (NCBI) | 3265 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for HRAS-Specific antibody 18295-1-AP | Download protocol |
IHC protocol for HRAS-Specific antibody 18295-1-AP | Download protocol |
IP protocol for HRAS-Specific antibody 18295-1-AP | Download protocol |
FC protocol for HRAS-Specific antibody 18295-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Transl Med Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS. | ||
Mol Cell KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis | ||
J Exp Med Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition | ||
Nat Commun A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Benjamin (Verified Customer) (01-10-2020) | Identified HRAS around 20kDa mark on western blot.
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