HRAS-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

HRAS-Specific Polyclonal Antibody for FC (Intra), IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

48

Reviews (1)

Cat no : 18295-1-AP

Synonyms

C BAS/HAS, C H RAS, C HA RAS1, CTLO, GTPase HRas, H Ras 1, H RASIDX, Ha Ras, HAMSV, HRAS, HRAS1, HRAS-Specific, K RAS, RASH1, Transforming protein p21

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Validation Data Gallery

• 

  WB analysis using 18295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18295-1-AP

  Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18295-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18295-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18295-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 18295-1-AP

  IP result of anti-HRAS-Specific(IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with mouse brain tissue lysate 1840 ug.

• 

  IHC staining of human kidney using 18295-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 18295-1-AP (HRAS-Specific Antibody) at dilution of 1:50.

• 

  FC experiment of HeLa using 18295-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HRAS-Specific (18295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells, SH-SY5Y cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat tissue
• Positive IP detected in: mouse brain tissue
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 42 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

18295-1-AP targets HRAS-Specific in WB, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-Ha-ras Harvey rat sarcoma viral oncogene homolog
• Calculated molecular weight: 21 kDa
• Observed molecular weight: 21 kDa
• GenBank accession number: BC006499
• Gene symbol: HRAS
• Gene ID (NCBI): 3265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS.; Authors - Sarah J Ross
human	WB	Sci Transl Med; The ERBB network facilitates KRAS-driven lung tumorigenesis.; Authors - Björn Kruspig
human	IP	Mol Cell; KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis; Authors - Hiroshi Senoo
human	WB	J Exp Med; Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition; Authors - Wei Wei
human	IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
human	WB	Nat Commun; A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS.; Authors - Nicolas Bery

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Benjamin (Verified Customer) (01-10-2020)

Identified HRAS around 20kDa mark on western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK

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