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HRAS-Specific Polyclonal antibody

HRAS-Specific Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 18295-1-AP

Synonyms

GTPase HRas, H RASIDX, HRAS, HRAS1, EC:3.6.5.2



Tested Applications

Positive WB detected inHEK-293 cells, SH-SY5Y cells, PC-3 cells, C6 cells, mouse kidney tissue, rat tissue, NIH/3T3 cells
Positive IP detected inmouse brain tissue, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18295-1-AP targets HRAS-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC006499
Gene Symbol HRAS
Gene ID (NCBI) 3265
RRIDAB_2121046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.

Protocols

Product Specific Protocols
WB protocol for HRAS-Specific antibody 18295-1-APDownload protocol
IF protocol for HRAS-Specific antibody 18295-1-APDownload protocol
IP protocol for HRAS-Specific antibody 18295-1-APDownload protocol
FC protocol for HRAS-Specific antibody 18295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Transl Med

Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS.

Authors - Sarah J Ross
humanWB

Sci Transl Med

The ERBB network facilitates KRAS-driven lung tumorigenesis.

Authors - Björn Kruspig
humanIP

Mol Cell

KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis

Authors - Hiroshi Senoo
humanWB

J Exp Med

Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition

Authors - Wei Wei
humanIF

Nat Commun

KRAS regulation by small non-coding RNAs and SNARE proteins.

Authors - Yonglu Che
humanWB

Nat Commun

A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS.

Authors - Nicolas Bery

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Benjamin (Verified Customer) (01-10-2020)

Identified HRAS around 20kDa mark on western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK