HRAS Polyclonal antibody

HRAS Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15531-1-AP

Synonyms

GTPase HRas, H RASIDX, HRAS1, EC:3.6.5.2, CTLO

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Validation Data Gallery

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  WB analysis of HeLa using 15531-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15531-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 15531-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 15531-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15531-1-AP

  IP result of anti-HRAS (IP:15531-1-AP, 4ug; Detection:15531-1-AP 1:300) with HeLa cells lysate 2600 ug.

• 

  IHC staining of mouse intestine using 15531-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 15531-1-AP (HRAS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 15531-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15531-1-AP (HRAS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, PC-12 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach tissue, mouse intestine; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

15531-1-AP targets HRAS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HRAS fusion protein Ag7704
• Full Name: v-Ha-ras Harvey rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC006499
• Gene Symbol: HRAS
• Gene ID (NCBI): 3265
• RRID: AB_2878147
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Med Rep; Sirtuin 2 knockdown inhibits cell proliferation and RAS/ERK signaling, and promotes cell apoptosis and cell cycle arrest in multiple myeloma.; Authors - Tianling Ding
human	WB	Naunyn Schmiedebergs Arch Pharmacol; The potential mechanism of Neu5Gc inducing colorectal cancer based on network pharmacology and experimental validation; Authors - Li-Chao Zhang
human	WB	Oncogene; Inhibition and degradation of NRAS with a pan-NRAS monobody; Authors - Michael Whaby