HPS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HPS1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, RIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15077-1-AP

Synonyms

HPS, Hermansky-Pudlak syndrome 1 protein, BLOC-3 complex member HPS1

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Validation Data Gallery

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  WB analysis of L02 using 15077-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15077-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 15077-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15077-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15077-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of L02 using 15077-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 15077-1-AP (HPS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 15077-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HPS1 antibody (15077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Y79 cells, Transfected HEK-293 cells, Raji cells, L02 cells, K-562 cells, mouse lung tissue
• Positive IF/ICC detected in: L02 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

15077-1-AP targets HPS1 in WB, IF/ICC, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPS1 fusion protein Ag7149
• Full Name: Hermansky-Pudlak syndrome 1
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 70-79 kDa
• GenBank Accession Number: BC000175
• Gene Symbol: HPS1
• Gene ID (NCBI): 3257
• RRID: AB_10694669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPS1, also named as Hermansky-Pudlak syndrome 1 protein, is a 700 amino acid protein, which is a component of multiple cytoplasmic organelles. HPS1 is Apparently crucial for their normal development and function and involved in intracellular protein sorting.HPS1 is involved in the biogenesis of early melanosomes.

Publications

Species	Application	Title
mouse	WB,IF	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook ; KO Validated
		Am J Respir Cell Mol Biol; Dysregulation of Galectin-3: Implications for Hermansky-Pudlak Syndrome Pulmonary Fibrosis.; Authors - Cullinane Andrew R AR
human	RIP	Clin Exp Metastasis; Long non-coding RNA NRSN2-AS1, transcribed by SOX2, promotes progression of esophageal squamous cell carcinoma by regulating the ubiquitin-degradation of PGK1.; Authors - Tongxin Xu
human	WB	Mol Genet Metab; In vitro functional correction of Hermansky-Pudlak Syndrome type-1 by lentiviral-mediated gene transfer.; Authors - Yasuhiro Ikawa
human	WB	J Cell Biol; Rab32 family proteins regulate autophagosomal components recycling; Authors - Zhe Wu