HOXA9 Polyclonal antibody

• Featured Product
• KD/KO Validated

HOXA9 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 18501-1-AP

Synonyms

HOX1G, HOX1.7, HOX1, Homeobox protein Hox-A9, Homeobox protein Hox-1G

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Validation Data Gallery

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  WB analysis using 18501-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18501-1-AP (HOXA9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse appendix using 18501-1-AP

  IP result of anti-HOXA9 (IP:18501-1-AP, 3ug; Detection:18501-1-AP 1:300) with mouse appendix tissue lysate 4000ug.

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  IHC staining of mouse skin using 18501-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18501-1-AP (HOXA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: EC109 cells
• Positive IP detected in: mouse appendix tissue
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• ChIP: See 2 publications below

Product Information

18501-1-AP targets HOXA9 in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOXA9 fusion protein Ag13350
• Full Name: homeobox A9
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC010023
• Gene Symbol: HOXA9
• Gene ID (NCBI): 3205
• RRID: AB_2861187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXA9, also known as HOX1G, belongs to the Abd-B homeobox family and transiently interacts with PRMT5 in TNF-alpha stimulated endothelial cells. HOXA9 is methylated on Arg-140 by PRMT5and methylation is critical for E-selectin induction. HOXA9 as a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. HOXA9 is required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. HOXA9 is 30 kDa, but methylation of HOXA9 is about 35-40 kDa. (PMID: 10082572)

Publications

Species	Application	Title
human	WB	Clin Epigenetics; Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line; Authors - Tina Aumer
human	WB	iScience; TIFAB accelerates MLL-AF9-Induced acute myeloid leukemia through upregulation of HOXA9.; Authors - Jinming Zhao
human	WB,chIP	Cell Signal; Aberrant TRPM4 expression in MLL-rearranged acute myeloid leukemia and its blockade induces cell cycle arrest via AKT/GLI1/Cyclin D1 pathway.; Authors - Fujue Wang ; KD Validated
human	WB	Cell Biosci; Hypoxic metabolism in human hematopoietic stem cells.; Authors - Fatih Kocabas
mouse	WB	Cell Rep; YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways.; Authors - Jiadong Liu
human	chIP	Sci Rep; Transcription Factor HOXA9 is Linked to the Calcification and Invasion of Papillary Thyroid Carcinoma.; Authors - Yilan Jin