HORMAD1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

HORMAD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7E12

Cat no : 67091-1-PBS

Synonyms

Cancer/testis antigen 46, CT46, DKFZp434A1315, HORMA domain containing 1, HORMAD1, Newborn ovary HORMA protein, NOHMA, RP11 363I22.1

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Validation Data Gallery

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  WB analysis of human testis using 67091-1-Ig (same clone as 67091-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 67091-1-Ig (HORMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 67091-1-Ig (same clone as 67091-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67091-1-Ig (HORMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67091-1-Ig (same clone as 67091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67091-1-Ig (same clone as 67091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67091-1-Ig (same clone as 67091-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67091-1-Ig (HORMAD1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67091-1-PBS in a different storage buffer formulation.

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Product Information

67091-1-PBS targets HORMAD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HORMAD1 fusion protein Ag28316
• Full Name: HORMA domain containing 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC047406
• Gene Symbol: HORMAD1
• Gene ID (NCBI): 84072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].