HORMAD1 Polyclonal antibody

HORMAD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 13917-1-AP

Synonyms

NOHMA, HORMAD 1, HORMA domain-containing protein 1, CT46

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Validation Data Gallery

• 

  WB analysis using 13917-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 13917-1-AP (HORMAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IF: See 20 publications below
• CoIP: See 1 publications below

Product Information

13917-1-AP targets HORMAD1 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HORMAD1 fusion protein Ag4914
• Full Name: HORMA domain containing 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC047406
• Gene Symbol: HORMAD1
• Gene ID (NCBI): 84072
• RRID: AB_2120844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734]. HORMAD1 exists as various isoform and the range of molecular weight of isoforms are sbout 36-45 kDa.

Publications

Species	Application	Title
mouse	IF	Sci Adv; SKP1 drives the prophase I to metaphase I transition during male meiosis.; Authors - Yongjuan Guan
human,mouse	WB,IF,CoIP	Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
mouse	IF	Nucleic Acids Res; NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.; Authors - Lu Chen
mouse	IF	Curr Biol; Prdm9 and Meiotic Cohesin Proteins Cooperatively Promote DNA Double-Strand Break Formation in Mammalian Spermatocytes.; Authors - Tanmoy Bhattacharyya
mouse	WB,IF	PLoS Genet; Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis.; Authors - Fukuda Tomoyuki T
mouse	WB,IF	PLoS Genet; Meiotic cohesins mediate initial loading of HORMAD1 to the chromosomes and coordinate SC formation during meiotic prophase.; Authors - Yasuhiro Fujiwara