HOPX Polyclonal antibody

• Featured Product
• KD/KO Validated

HOPX Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

41

Reviews (3)

Cat no : 11419-1-AP

Synonyms

CAMEO, HOD, Homeodomain only protein, HOP, HOP homeobox, HOPX, LAGY, NECC1, OB1, Odd homeobox protein 1, SMAP31, TOTO

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Validation Data Gallery

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  WB analysis using 11419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11419-1-AP (HOPX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11419-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11419-1-AP (HOPX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 11419-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse small intestine tissue, rat lung tissue
• Positive IHC detected in: human placenta tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 9 publications below
• IF: See 25 publications below

Product Information

11419-1-AP targets HOPX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOPX fusion protein Ag1979
• Full Name: HOP homeobox
• Calculated Molecular Weight: 73 aa, 8 kDa
• Observed Molecular Weight: 8-12 kDa
• GenBank Accession Number: BC014225
• Gene Symbol: HOPX
• Gene ID (NCBI): 84525
• RRID: AB_10693525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOPX (Homeodomain-only protein) gene has various synonyms including HOD, HOP, OB1, LAGY and NECC1. The protein encoded by this gene is an unusual homeodomain protein that lacks certain conserved residues required for DNA binding. HOPX has diverse effects on cardiac growth. Manipulation of Hopx function in murine models is associated with cardiac hypertrophy, dilation and fibrosis. HOPX protein acts as an antagonist to the serum response factor (SRF), which regulates the opposing processes of cell proliferation and differentiation. Overexpression of HOPX causes cardiac hypertrophy. HOPX protein can inhibit SRF-dependent transcriptional activation by recruiting histone deacetylase (HDAC) activity.

Publications

Species	Application	Title
human	IHC	Nature; Cell stress in cortical organoids impairs molecular subtype specification.; Authors - Aparna Bhaduri
human	IF	Cell; Origins and Proliferative States of Human Oligodendrocyte Precursor Cells.; Authors - Wei Huang
human	IHC	Cancer Cell; Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma.; Authors - Lindsay M LaFave
human		Cancer Cell; Control of alveolar differentiation by the lineage transcription factors GATA6 and HOPX inhibits lung adenocarcinoma metastasis.; Authors - William K C Cheung
human	IF	Cell Stem Cell; LIF signaling regulates outer radial glial to interneuron fate during human cortical development; Authors - Madeline G Andrews
mouse	IF	Protein Cell; BMP7 expression in mammalian cortical radial glial cells increases the length of the neurogenic period; Authors - Zhenmeiyu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nada (Verified Customer) (12-13-2024)

Performs well for immunoblotting and flow cytometry (performed internal validation prior to using)

• Applications: Western Blot, Flow Cytometry

FH

Ana (Verified Customer) (11-10-2020)

We recently published with this paper: PubMed ID: 32819430 Images are within the manuscript.Protocol information: We used Citirc Acid Buffer Antigen Retrieval

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Human Brain (fetal), Paraffin Processed

HOPX Antibody Immunohistochemistry validation (1:250 dilution) in Human Brain (fetal), Paraffin Processed (Cat no:11419-1-AP)

FH

Nicole (Verified Customer) (07-23-2019)

Used to mark stem cell cultures for differentiation into neural like cells

• Applications: Immunohistochemistry, Immunofluorescence,
• Cell Tissue Type: Stem Cell culture