HOMER3 Polyclonal antibody

• Featured Product
• KD/KO Validated

HOMER3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16624-1-AP

Synonyms

HOMER 3, homer homolog 3 (Drosophila), Homer protein homolog 3, HOMER3, VESL3

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Validation Data Gallery

• 

  WB analysis of mouse brain using 16624-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16624-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16624-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16624-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16624-1-AP (HOMER3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cerebellum using 16624-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 16624-1-AP (HOMER3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 16624-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 16624-1-AP (HOMER3 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse lung tissue, human brain tissue, rat brain tissue
• Positive IHC detected in: human cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1200
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16624-1-AP targets HOMER3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOMER3 fusion protein Ag9923
• Full Name: homer homolog 3 (Drosophila)
• Calculated Molecular Weight: 361 aa, 40 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC012113
• Gene Symbol: HOMER3
• Gene ID (NCBI): 9454
• RRID: AB_2121013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOMER3 is a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.

Publications

Species	Application	Title
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
rat	WB	Neuroscience; Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety.; Authors - Dan Liu
human	IHC	Cell Death Dis; Long non-coding RNA HOMER3-AS1 drives hepatocellular carcinoma progression via modulating the behaviors of both tumor cells and macrophages.; Authors - Jian Pu ; KD Validated