HOMER2 Polyclonal antibody

HOMER2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11143-1-AP

Synonyms

Homer protein homolog 2, homer homolog 2 (Drosophila), HOMER 2, Cupidin, CPD

---

Validation Data Gallery

• 

  WB analysis using 11143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11143-1-AP (HOMER2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11143-1-AP (HOMER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 11143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11143-1-AP (HOMER2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 11143-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11143-1-AP (HOMER2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 11143-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HOMER2 antibody (11143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HEK-293T cells, MCF-7 cells, rat kidney tissue, LnCaP cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11143-1-AP targets HOMER2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOMER2 fusion protein Ag1606
• Full Name: homer homolog 2 (Drosophila)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC012109
• Gene Symbol: HOMER2
• Gene ID (NCBI): 9455
• RRID: AB_2877747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOMER2 (Homer Scaffold Protein 2), a postsynaptic density scaffolding protein, is essential for normal hearing in humans and mice(PMID: 25816005). HOMER2 negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway(Uniprot). HOMER2 belongs to the homer family of dendritic proteins which are immediate early gene products who bind to group 1 metabotropic glutamate receptors (mGluRs), proteins associated to triggering intracellular calcium release. Additionally, HOMER2 connects mGluRs to the inositol triphosphate receptors (IP3R).

Publications

Species	Application	Title
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin