HOMER1 Recombinant antibody

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  WB analysis using 85692-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85692-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 85692-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 85692-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85692-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85692-4-RR against Human HOMER1 were performed. The affinity constant is 0.309 nM.

HOMER1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 85692-4-RR

Clone No.243007G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, Homer-1

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Tested Applications

• Positive WB detected in: LNCaP cells, mouse brain tissue, MOLT-4 cells, HEK-293 cells, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85692-4-RR targets HOMER1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HOMER1 fusion protein Ag3109
• Full Name: homer homolog 1 (Drosophila)
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 46-48 kDa
• GenBank Accession Number: BC015502
• Gene Symbol: HOMER1
• Gene ID (NCBI): 9456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86YM7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.